The Biolit 2 is a compact, air-cooled femtosecond laser for multiphoton microscopy and bio-imaging, offering 2 W power and 100 nJ pulses at 1050 nm. Its exceptional beam quality, with an M2 of 1.05 and circularity of 0.94, meets the rigorous demands of advanced tissue imaging and multiphoton polymerization. Featuring clean, Gaussian-like 55 fs pulses and a dispersion pre-compensation function for optimal delivery through complex systems, it supports a 15 - 40 MHz pulse repetition range, tailored for enhanced imaging quality.